Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse CCR2 Antibody

Detection of CCR2 in NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP by Flow Cytometry.

NS0 mouse myeloma cell line transfected with either (A) mouse CCR2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse/Rat CCR2 Monoclonal Antibody (Catalog # MAB55381) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). Quadrant markers were set based on isotype control (Rat IgG_2B,Catalog # MAB0061; data not shown). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CCR2 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB55381 in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Gene Symbol

CCR2

Additional CCR2 Products

Product Documents for Mouse CCR2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CCR2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CCR2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB55381

Key Product Details

Species Reactivity

Validated:

Cited:

Applications