Mouse CCR2 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB5538P
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Mass Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 475301
Product Specifications
Immunogen
L1.2 mouse pro-B cell line transfected with mouse CCR2
Specificity
Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse CCR2 PE-conjugated Antibody
Detection of CCR2 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CCR2 PE-conjugated Monoclonal Antibody (Catalog # FAB5538P) and Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F). Quadrant marker was set based on isotype control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse CCR2 by Flow Cytometry
Depletion of CCR2 cells enhances tumor radiosensitivity. a Scheme of treatment. b Treated tumor was measured every 3-4 days for 33 days starting from the day of radiation (n = 10). c Percentage of mice still bearing tumor at the end of the treatments (n = 10), P < 0.05. d Flow cytometry profile of CCR2+Ly6Chi in tumors 3 days post the start of treatments. Left, representative flow graph, gated on CD45+CD11b+cells; Middle, percentage of CD45+; Right, absolute number of cells (n = 4). NS, non-significant.*P < 0.05; **P < 0.01; ***P < 0.001. Each experiment was repeated 3 times. Data are presented as mean ± s.e.m Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29170400), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse CCR2 by Flow Cytometry
MDSC depletion enhances tumor response to radiation and STING agonist cGAMP treatment. a Flow cytometry analysis of immune CCR2+Ly6chi cells 3 days after IR (n = 3). b Tumor growth curves; n = 6; c percentage of tumor bearing mice ending on 33 days after starting of the treatments. d, e ratios of percentage of CD8 over CCR2+Ly6chi and Treg cells in treated tumors, respectively. n = 3. *P < 0.05; **P < 0.01. The experiments were repeated three times. Data are presented as mean ± s.e.m Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29170400), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse CCR2 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.3 using FAB5538P in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR2
Alternate Names
CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R
Gene Symbol
CCR2
Additional CCR2 Products
Product Documents for Mouse CCR2 PE-conjugated Antibody
Product Specific Notices for Mouse CCR2 PE-conjugated Antibody
For research use only
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