Mouse CD117/c-kit Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM1356

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

MAB1356

Alexa Fluor 350

FAB1356U

Alexa Fluor 405

FAB1356V

Alexa Fluor 488

FAB1356G

Alexa Fluor 532

FAB1356X

Alexa Fluor 594

FAB1356T

Alexa Fluor 647

FAB1356R

Alexa Fluor 700

FAB1356N

Alexa Fluor 750

FAB1356S

Allophycocyanin

FAB1356A

Allophycocyanin/Cy7

FAB1356APCCY7

CoraFluor 1

FAB1356CL1

DyLight 350

FAB1356D

DyLight 405

FAB1356E

DyLight 488

FAB1356K

DyLight 550

FAB1356L

DyLight 594

FAB1356M

DyLight 650

FAB1356W

DyLight 680

FAB1356Y

DyLight 755

FAB1356Z

FITC

FAB1356F

HRP

FAB1356H

Janelia Fluor 525

FAB1356JF525

Janelia Fluor 549

FAB1356I

Janelia Fluor 585

FAB1356JF585

Janelia Fluor 635

FAB1356JF635

Janelia Fluor 646

FAB1356J

Janelia Fluor 669

FAB1356JF669

mFluor Violet 450 SE

FAB1356MFV450

mFluor Violet 500 SE

FAB1356MFV500

mFluor Violet 610 SE

FAB1356MFV610

PE/Atto594

FAB1356PEATT594

PE/Cy5.5

FAB1356PECY55

PE/Cy7

FAB1356PECY7

PerCP

FAB1356C

Phycoerythrin

FAB1356P

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Western Blot

Cited:

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Rat IgG_2A Clone # 180627

View all CD117/c-kit Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Met1-Thr519
Accession # P05532

Specificity

Detects mouse CD117/c-kit in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Applications for Mouse CD117/c-kit Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Lineage depleted mouse bone marrow

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cerebellum)

Western Blot

1 µg/mL
Sample: Recombinant Mouse CD117/c-kit under non-reducing conditions only

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Gene Symbol

KIT

UniProt

P05532

Additional CD117/c-kit Products

Product Documents for Mouse CD117/c-kit Biotinylated Antibody

Product Datasheets

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COA

SDS

Product Specific Notices for Mouse CD117/c-kit Biotinylated Antibody

For research use only

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Reviews

Calculators
Flow Cytometry