Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE-conjugated Monoclonal Antibody (Catalog # FAB1356P) and Rat Anti-Mouse Sca-1/Ly6 APC-conjugated Monoclonal Antibody (Catalog # FAB1226A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD117/c-kit PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse splenocytes gated on hematopoietic lineage negative cells

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD117/c-kit

SCF R, also known as c-kit proto-oncogene protein and CD117, is a 145-160 kDa member of the type III tyrosine kinase family of proteins (1,2). It is expressed on hematopoietic and endothelial cell progenitors, mast cells, germ cells, melanocytes, trigeminal neurons, glial cells, placenta, kidney, lung, and gut. SCF R is a type I transmembrane glycoprotein that undergoes splicing during spermatogenesis. Two isoforms of 50 kDa and 30 kDa are generated that represent C-terminal intracellular functional products (3). SCF R also undergoes cleavage by select cell types, generating a 95-100 kDa soluble form (4). Over amino acids 25-519, mouse SCF R shares 73% and 87% amino acid sequence identity with human and rat SCF R, respectively.

References

Heldin, C-H. and J. Lennartsson (2013) Cold Spring Harb. Perspect. Biol. 5:a009100.
Lennartsson, J. and L. Ronnstrand (2012) Physil. Rev. 92:1619.
Zhang, L. et al. (2013) BMC Dev. Biol. 13:38.
Broudy, V.C. et al. (2001) Cytokine 15:188.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Gene Symbol

KIT

UniProt

P05532

Additional CD117/c-kit Products

Product Documents for Mouse CD117/c-kit PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD117/c-kit PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse CD117/c-kit PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1356P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD117/c-kit Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD117/c-kit PE-conjugated Antibody

Detection of CD117/c-kit in Mouse Splenoctyes by Flow Cytometry.