Mouse CD155/PVR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6909
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 690912
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD155/PVR
Asp29-Leu348
Accession # NP_081790
Asp29-Leu348
Accession # NP_081790
Specificity
Detects mouse CD155/PVR in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CD155/PVR Antibody
Detection of Mouse CD155/PVR by Western Blot.
Western blot shows lysates of TM4 mouse sertoli cell line and mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD155/PVR at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.CD155/PVR in Mouse Thymocytes.
CD155/PVR was detected in immersion fixed adult mouse thymocytes using Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Detection of CD155/PVR in Mouse Thymocytes by Flow Cytometry
Mouse thymocytes were stained with Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).Applications for Mouse CD155/PVR Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA binding assay, 0.2-0.8 μg/mL of this
antibody will block 50% of the binding of
Recombinant Mouse CD155/PVR Fc Chimera (Catalog # 9670-CD) to immobilized Recombinant
Mouse
CD96 (Catalog # 5690-CD)
coated at 1 μg/mL (100 μL/well). At 10 μg/mL, this antibody will
block >90% of the binding.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse thymocytes
Sample: Mouse thymocytes
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed adult mouse thymocytes
Sample: Immersion fixed adult mouse thymocytes
Western Blot
2 µg/mL
Sample: TM4 mouse sertoli cell line and mouse small intestine tissue
Sample: TM4 mouse sertoli cell line and mouse small intestine tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD155/PVR
Long Name
Poliovirus Receptor
Alternate Names
CD155, HVED, Necl-5, PVR, PVS
Entrez Gene IDs
Gene Symbol
PVR
UniProt
Additional CD155/PVR Products
Product Documents for Mouse CD155/PVR Antibody
Product Specific Notices for Mouse CD155/PVR Antibody
For research use only
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