Western blot shows lysates of TM4 mouse sertoli cell line and mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD155/PVR at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

CD155/PVR in Mouse Thymocytes.

CD155/PVR was detected in immersion fixed adult mouse thymocytes using Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of CD155/PVR in Mouse Thymocytes by Flow Cytometry

Mouse thymocytes were stained with Rat Anti-Mouse CD155/PVR Monoclonal Antibody (Catalog # MAB6909, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Applications for Mouse CD155/PVR Antibody

Application

Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA binding assay, 0.2-0.8 μg/mL of this antibody will block 50% of the binding of Recombinant Mouse CD155/PVR Fc Chimera (Catalog # 9670-CD) to immobilized Recombinant Mouse CD96 (Catalog # 5690-CD) coated at 1 μg/mL (100 μL/well). At 10 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Mouse thymocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed adult mouse thymocytes

Western Blot

2 µg/mL
Sample: TM4 mouse sertoli cell line and mouse small intestine tissue

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor) and Necl-5 (Nectin-like molecule-5), is a 70 kDa type I transmembrane glycoprotein that binds poliovirus as well as Vitronectin, Nectin-3, and DNAM-1. CD155 contains three Ig-like domains in its extracellular region. It is expressed in a variety of normal tissues and is upregulated in tumor cells of epithelial and neuronal origin. Within aa 29-348 of the extracellular region, mouse CD155 shares 45% and 73% aa sequence identity with human and rat CD155, respectively.

Long Name

Poliovirus Receptor

Alternate Names

CD155, HVED, Necl-5, PVR, PVS

Entrez Gene IDs

5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)

Gene Symbol

PVR

UniProt

NP_081790

Additional CD155/PVR Products

Product Documents for Mouse CD155/PVR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD155/PVR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CD155/PVR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6909

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD155/PVR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD155/PVR Antibody

Detection of Mouse CD155/PVR by Western Blot.