Mouse CD300g/Nepmucin Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF5554
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD300g/Nepmucin
Leu19-Ile284
Accession # NP_001154183
Leu19-Ile284
Accession # NP_001154183
Specificity
Detects mouse CD300g/Nepmucin in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CD300g/Nepmucin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse CD300g/Nepmucin Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CD300g/Nepmucin
Sample: Recombinant Mouse CD300g/Nepmucin
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD300g/Nepmucin
Nepmucin (a not expressed in Peyer's Patch mucin; also CLM-9, CD300g and TREM4) is an 80‑95 kDa member of the CD300L family of proteins. It is expressed on endothelium and serves multiple functions. It is known to form homotypic interactions between endothelial cells, bind lymphocytes via its Ig-like and mucin domains and serve as a transcellular transport vehicle for IgA and IgM. Mature mouse Nepmucin is a 397 amino acid (aa) type I transmembrane glycoprotein. Its extracellular region (aa 19‑288) contains one V-type Ig-like domain (aa 19‑124) plus an O-glycosylated mucin region (aa 136‑276). There are three isoform variants. One is 60‑75 kDa in size and shows an Ala substitution for aa 126‑170, a second is 46‑50 kDa in size and shows an Arg substitution for aa 170‑254, while a third is 36 kDa in size and shows a SerTyr substitution for aa 134‑256. Over aa 1‑284, mouse Nepmucin shares 89% and 53% aa identity with rat and human Nepmucin, respectively.
Alternate Names
CD300LG, CLM9, Nepmucin, TREM-4
Gene Symbol
CD300LG
UniProt
Additional CD300g/Nepmucin Products
Product Documents for Mouse CD300g/Nepmucin Biotinylated Antibody
Product Specific Notices for Mouse CD300g/Nepmucin Biotinylated Antibody
For research use only
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