Mouse CD300g/Nepmucin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5554
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD300g/Nepmucin
Leu19-Ile284
Accession # NP_001154183
Leu19-Ile284
Accession # NP_001154183
Specificity
Detects mouse CD300g/Nepmucin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human CD300g/Nepmucin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse CD300g/Nepmucin Antibody
Detection of Mouse CD300g/Nepmucin by Western Blot.
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD300g/Nepmucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5554) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD300g/Nepmucin at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse CD300g/Nepmucin Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse kidney tissue
Sample: Mouse kidney tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD300g/Nepmucin
Nepmucin (a not expressed in Peyer's Patch mucin; also CLM-9, CD300g and TREM4) is a member of the CD300L family of proteins. It is expressed on endothelium and serves multiple functions. It is known to form homotypic interactions between endothelial cells, bind lymphocytes via its Ig-like and mucin domains and serve as a transcellular transport vehicle for IgA and IgM. Mature mouse Nepmucin is a 397 amino acid (aa) type I transmembrane glycoprotein. Its extracellular region (aa 19‑288) contains one V-type Ig-like domain (aa 19‑124) plus an O-glycosylated mucin region (aa 136‑276). There are at least three isoform variants. One is 60‑75 kDa in size and shows an Ala substitution for aa 126‑170, a second is 46‑50 kDa in size and shows an Arg substitution for aa 170‑254, while a third is 36 kDa in size and shows a SerTyr substitution for aa 134‑256. Over aa 1‑284, mouse Nepmucin shares 89% and 53% aa identity with rat and human Nepmucin, respectively.
Alternate Names
CD300LG, CLM9, Nepmucin, TREM-4
Gene Symbol
CD300LG
UniProt
Additional CD300g/Nepmucin Products
Product Documents for Mouse CD300g/Nepmucin Antibody
Product Specific Notices for Mouse CD300g/Nepmucin Antibody
For research use only
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