Mouse CD302/CLEC13A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6424
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD302/CLEC13A
Asp21-His165
Accession # Q9DCG2
Asp21-His165
Accession # Q9DCG2
Specificity
Detects mouse CD302/CLEC13A in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human CD302 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse CD302/CLEC13A Antibody
Detection of Mouse CD302/CLEC13A by Western Blot.
Western blot shows lysates of mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CD302/CLEC13A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6424) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD302/CLEC13A at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse CD302/CLEC13A Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse spleen tissue
Sample: Mouse spleen tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD302/CLEC13A
CD302 (also DCL-1 and CLEC13A) is a 31-36 kDa member of the C-type lectin domain family of proteins. It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages. CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion and migration. Mature mouse CD302 is a 208 amino acid (aa) type I transmembrane glycoprotein. It contains a 146 aa extracellular domain (ECD) (aa 21-165) plus a 42 aa cytoplasmic region. The ECD possesses one C-type lectin domain (aa 30-149) that apparently cannot bind carbohydrate. In human, CD302 has also been detected in dendritic cells as the C-terminal portion of a 215 kDa fusion protein that involves DEC205. CD302 contributes aa 23-232 to this fusion protein. Mouse CD302 has two potential splice variants. One shows an alternative start site at Met61, while another shows a deletion of aa 154-162. Over aa 21-165, mouse CD302 shares 82% and 92% aa identity with human and rat CD302, respectively.
Alternate Names
BIMLEC, CD302, CLEC13A, DCL1
Gene Symbol
CD302
UniProt
Additional CD302/CLEC13A Products
Product Documents for Mouse CD302/CLEC13A Antibody
Product Specific Notices for Mouse CD302/CLEC13A Antibody
For research use only
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