Mouse CD68/SR-D1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10114
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2449C
Product Specifications
Immunogen
Chinese Hamster Ovary cell line, CHO-derived mouse CD68
Asp21-Pro282
Accession # P31996
Asp21-Pro282
Accession # P31996
Specificity
Detects mouse CD68 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Mouse CD68/SR-D1 Antibody
CD68/SR‑D1 in RAW 264.7 and NIH-3T3 Mouse Cell Lines.
CD68/SR-D1 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line (positive stain) and NIH-3T3 mouse embryonic fibroblast cell line (negative stain) using Rabbit Anti-Mouse CD68/SR-D1 Monoclonal Antibody (Catalog # MAB10114) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of CD68 in Raw264.7 Mouse Cell line by Flow Cytometry.
Raw264.7 mouse cell line was stained with Rabbit Anti-Mouse CD68 Monoclonal Antibody (Catalog # MAB10114, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1x) (Catalog # FC004) and permeabilized with ice-cold methanol. View our protocol for Staining Intracellular Molecules.Applications for Mouse CD68/SR-D1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed RAW264.7 mouse monocyte/macrophage cell line
Sample: Immersion fixed RAW264.7 mouse monocyte/macrophage cell line
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: RAW264.7 mouse monocyte/macrophage cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with ice-cold methanol.
Sample: RAW264.7 mouse monocyte/macrophage cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with ice-cold methanol.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD68/SR-D1
Alternate Names
CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1
Gene Symbol
CD68
UniProt
Additional CD68/SR-D1 Products
Product Documents for Mouse CD68/SR-D1 Antibody
Product Specific Notices for Mouse CD68/SR-D1 Antibody
For research use only
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