Mouse CD79A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6968
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD79A
Leu29-Arg137
Accession # P11911
Leu29-Arg137
Accession # P11911
Specificity
Detects mouse CD79A in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human CD79A is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse CD79A Antibody
Detection of Mouse CD79A by Western Blot.
Western blot shows lysates of A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD79A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6968) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD79A at approximately 32-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse CD79A Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A20 mouse B cell lymphoma cell line
Sample: A20 mouse B cell lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD79A
CD79A (also known as Mb-1, Ig alpha and B cell antigen receptor complex-associated protein alpha-chain) is a 30-40 kDa member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with CD79B. This complex interacts noncovalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79A:B initiates signaling. CD79A is essential for the differentiation of pre-B cells, and the pre-BCR regulates the surface expression of IL-7R. Mature mouse CD79A is a 192 amino acid (aa) type I transmembrane glycoprotein (aa 29-220). It contains an extracellular region (aa 29-137) with one C2-type Ig-like domain (aa 29-117), and an ITAM-containing cytoplasmic domain (aa 171-199). Mouse CD79A and CD79B share only 22% aa identity. Over aa 29-137, mouse CD79A shares 57% and 80% aa identity with human and rat CD79A, respectively.
Alternate Names
CD79A, MB-1
Gene Symbol
CD79A
UniProt
Additional CD79A Products
Product Documents for Mouse CD79A Antibody
Product Specific Notices for Mouse CD79A Antibody
For research use only
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