Mouse CD8 alpha Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB116F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 53-6.7
Product Specifications
Immunogen
Mouse thymus or spleen
Specificity
Detects both the alpha and alpha' chains of mouse CD8 (1).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CD8 alpha Fluorescein-conjugated Antibody
Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD8a Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB116F) or (B) Rat IgG2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse CD8 alpha Fluorescein-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using FAB116F in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD8
CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha/Ly-2 or alpha′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma delta TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).
References
- Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
- Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
- Hayakawa, K. et al. (1994) Science 263:1131.
- MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
- Rocha, B. et al. (1992) Immunol. Today 13:449.
- Wang, J. and J.R. Klein (1994) Science 265:1860.
- Vermec, D. et al. (1992) J. Exp. Med. 176:47.
- Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.
Alternate Names
CD8, CD8A
Entrez Gene IDs
Gene Symbol
CD8A
Additional CD8 Products
Product Documents for Mouse CD8 alpha Fluorescein-conjugated Antibody
Product Specific Notices for Mouse CD8 alpha Fluorescein-conjugated Antibody
For research use only
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