Mouse CD90/Thy1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7335
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD90/Thy1
Gln20-Lys130
Accession # P01831
Gln20-Lys130
Accession # P01831
Specificity
Detects mouse CD90/Thy1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human CD90/Thy1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse CD90/Thy1 Antibody
Detection of Mouse CD90/Thy1 by Western Blot.
Western blot shows lysates of mouse thymus tissue, mouse brain tissue, EL-4 mouse lymphoblast cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7335) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD90/Thy1 at approximately 25-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse CD90/Thy1 Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: Mouse thymus tissue, mouse brain tissue, EL‑4 mouse lymphoblast cell line, and CTLL‑2 mouse cytotoxic T cell line
Sample: Mouse thymus tissue, mouse brain tissue, EL‑4 mouse lymphoblast cell line, and CTLL‑2 mouse cytotoxic T cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD90/Thy1
Alternate Names
CD90, Thy1
Gene Symbol
THY1
UniProt
Additional CD90/Thy1 Products
Product Documents for Mouse CD90/Thy1 Antibody
Product Specific Notices for Mouse CD90/Thy1 Antibody
For research use only
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