Mouse CXCL10/IP-10/CRG-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-466-NA
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Rat
Applications
Validated:
Neutralization, Western Blot
Cited:
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Tissue Culture, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CXCL10/IP-10/CRG-2
Ile22-Pro98
Accession # P17515
Ile22-Pro98
Accession # P17515
Specificity
Detects mouse CXCL10/IP-10/CRG-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human CXCL10 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CXCL10/IP-10/CRG-2 Antibody
Detection of Mouse CXCL10/IP‑10/CRG‑2 by Western Blot.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CXCL10/IP-10/CRG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL10/IP-10/CRG-2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chemotaxis Induced by CXCL10/CRG‑2 and Neutral-ization by Mouse CXCL10/ CRG‑2 Antibody.
Recombinant Mouse CXCL10/ CRG-2 (Catalog # 466-CR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL10/ CRG-2 (0.5 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Mouse CXCL10/CRG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA). The ND50 is typically 5-25 µg/mL.
Detection of Mouse CXCL10/IP-10/CRG-2 by Western Blot
Conditional deletion of astroglial CXCL10. (A) Location of loxP sites in the CXCL10 locus in CXCL10fl/fl mice. (B) Day 17 post-MOG peptide injection (dpi) representative images of immunostaining for CXCL10-Red, GFAP-Green, and DAPI-Blue. (C) Quantification of images for CXCL10 integrated density at 17 dpi (n = 4–7 mice/group). (D and E) qRT/PCR for CXCL10 and CXCL9 in spinal cord of astroglial CXCL10 knockout and littermate control mice at 17 dpi (n = 6 mice/group). (F) Quantification of CXCL10 protein levels in the spinal cord of astroglial CXCL10 knockout and control mice at 14 dpi, by western blot analysis (n = 4 mice/group). (G) Data were normalized to GAPDH, P = 0.046. Vertical bars = SEMs. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24924222), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse CXCL10/IP-10/CRG-2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Neutralization
Measured by its ability to neutralize CXCL10/IP-10/CRG-2-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 5-25 µg/mL in the presence of 0.5 µg/mL Recombinant Mouse CXCL10/IP-10/CRG-2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 2.7 using AF-466-NA in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL10/IP-10/CRG-2
References
- Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
- Vanguri, P. (1996) J. Neuroimmunol. 56:35.
- Sgadari, C. et al. (1996) Blood 87:3877.
Alternate Names
CRG-2, CRG2, IP-10
Gene Symbol
CXCL10
UniProt
Additional CXCL10/IP-10/CRG-2 Products
Product Documents for Mouse CXCL10/IP-10/CRG-2 Antibody
Product Specific Notices for Mouse CXCL10/IP-10/CRG-2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...