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Mouse CXCL11/I-TAC Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF572

R&D Systems, part of Bio-Techne
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BAF572

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA Detection (Matched Antibody Pair), Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCL11/I-TAC
Phe22-Met100
Accession # Q9JHH5

Specificity

Detects mouse CXCL11/I-TAC in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant human CCL11, recombinant mouse (rm) BLC, rmCRG-2, rmGCP-2, rmMIG, rmPF4, and rmSDF-1 alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse CXCL11/I-TAC Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CXCL11/I-TAC (Catalog # 572-MC)

Mouse CXCL11/I-TAC Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse CXCL11/I-TAC Antibody (Catalog # AF572)
  • Standard: Recombinant Mouse CXCL11/I-TAC Protein (Catalog # 572-MC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL11/I-TAC

CXCL11 (also known as I-TAC, SCYB9B, H174, IP-9, and beta-R1) is a member within the non-ELR CXC chemokine subgroup and has been designated CXCL11. CXCL11, together with MIG and IP-10, constitute a subset of chemokines that are ligands for CXCR3, a chemokine receptor that is primarily expressed on activated Th1 cells and NK cells. The three chemokines were also reported to act as antagonists for CCR3, a chemokine receptor that is preferentially expressed on activated Th2 cells. Mouse CXCL11 cDNA encodes a 100 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to yield a 79 aa residue mature protein. Mature mouse and human CXCL11 share 71% aa sequence identity. Mouse CXCL11 also shares 36% and 29% aa sequence identity with mouse IP-10 (CRG-2) and mouse MIG, respectively. The gene for mouse CXCL11 has been mapped to chromosome 5, in close proximity to the IP-10 and MIG genes. Mouse CXCL11 is induced in multiple tissues during endoxemia, with the greatest expression in lung, heart, small intestine, and kidney. The endotoxemia-induced mouse CXCL11 expression is strongly attenuated by treatment with glucocorticoid.

References

  1. Widney, D.P. et al. (2000) J. Immunol. 164:6322.
  2. Meyer, M. et al. (2000) Cytogenet. Cell Genet. 88:278.
  3. Loetscher, P. et al. (2001) J. Biol. Chem. Manuscript M005652200.

Alternate Names

beta-R1, H174, I-TAC, ITAC, SCYB9B

Entrez Gene IDs

6373 (Human); 56066 (Mouse)

Gene Symbol

CXCL11

UniProt

Additional CXCL11/I-TAC Products

Product Documents for Mouse CXCL11/I-TAC Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CXCL11/I-TAC Biotinylated Antibody

For research use only

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