Mouse Dkk-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1765
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 259631
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Dkk-1
Ser30-His272
Accession # O54908
Ser30-His272
Accession # O54908
Specificity
Detects mouse Dkk-1 in direct ELISAs and Western blots. Does not cross-react with recombinant human Dkk-1, -3, or -4.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Dkk-1 Antibody
Dkk-1 in Mouse Embryo.
Dkk-1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c) using Mouse Dkk-1 Monoclonal Antibody (Catalog # MAB1765) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in lung. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Dkk-1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5-15.5)
Sample: Immersion fixed frozen sections of mouse embryo (E13.5-15.5)
Western Blot
1 µg/mL
Sample: Recombinant Mouse Dkk-1 (Catalog # 1765-DK)
Recombinant Mouse Dkk-1 (Catalog # 5897-DK)
Sample: Recombinant Mouse Dkk-1 (Catalog # 1765-DK)
Recombinant Mouse Dkk-1 (Catalog # 5897-DK)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dkk-1
Long Name
Dickkopf-1
Alternate Names
Dkk1
Gene Symbol
DKK1
UniProt
Additional Dkk-1 Products
Product Documents for Mouse Dkk-1 Antibody
Product Specific Notices for Mouse Dkk-1 Antibody
For research use only
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