Mouse Endosialin/CD248 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7535
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 458606
Product Summary for Mouse Endosialin/CD248 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Endosialin/CD248
Val13-Arg693 (predicted)
Accession # Q91V98
Val13-Arg693 (predicted)
Accession # Q91V98
Specificity
Detects mouse Endosialin/CD248 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with
recombinant human Endosialin/CD248 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Endosialin/CD248 Antibody
Endosialin/CD248 in bEnd.3 Mouse Cell Line.
Endosialin/CD248 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse Endosialin/CD248 Monoclonal Antibody (Catalog # MAB7535) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Endosialin/CD248 in IMR-32 Human Cell Line.
Endosialin/CD248 was detected in immersion fixed IMR-32 human neuroblastoma cell line using Rat Anti-Mouse Endosialin/CD248 Monoclonal Antibody (Catalog # MAB7535) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse Endosialin/CD248 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed bEnd.3 mouse endothelioma cell line and IMR-32 human neuroblastoma cell line
Sample: Immersion fixed bEnd.3 mouse endothelioma cell line and IMR-32 human neuroblastoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endosialin/CD248
Alternate Names
CD164L1, CD248, TEM1
Gene Symbol
CD248
UniProt
Additional Endosialin/CD248 Products
Product Documents for Mouse Endosialin/CD248 Antibody
Product Specific Notices for Mouse Endosialin/CD248 Antibody
For research use only
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