Mouse FGF-21 Antibody

Detection of Mouse FGF‑21 by Western Blot.

Western blot shows lysates of mouse spleen tissue and rat spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3057) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF-21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse FGF-21 by Western Blot

Increased cellular stress response in muscle of DTG mice.(A) Gene expression analysis of FGF21 in Quadriceps muscle by quantitative RT-PCR (n = 8 per group) and (B) FGF21 plasma concentration of 12-wk-old WT, DN-AMPK alpha2, UCP1-TG and DTG mice (n = 9–10 per group). (B) Representative western blots of proteins involved in integrated stress response and FGF21 induction and (C) of cellular stress response markers HSP25 and HSP70 in Quadriceps muscle from 12-wk-old WT, DN-AMPK alpha2, UCP1-TG and DTG mice, Mitofusin-2 (MFN2) was used as a loading control (n = 2 out of 6–8 analyzed per group). Means with different letters are significantly different (1way ANOVA and Bonferroni's multiple comparisons test, p<0.05). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0094689), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Mouse FGF-21 Antibody by Western Blot

Increased cellular stress response in muscle of DTG mice.(A) Gene expression analysis of FGF21 in Quadriceps muscle by quantitative RT-PCR (n = 8 per group) and (B) FGF21 plasma concentration of 12-wk-old WT, DN-AMPK alpha2, UCP1-TG and DTG mice (n = 9–10 per group). (B) Representative western blots of proteins involved in integrated stress response and FGF21 induction and (C) of cellular stress response markers HSP25 and HSP70 in Quadriceps muscle from 12-wk-old WT, DN-AMPK alpha2, UCP1-TG and DTG mice, Mitofusin-2 (MFN2) was used as a loading control (n = 2 out of 6–8 analyzed per group). Means with different letters are significantly different (1way ANOVA and Bonferroni's multiple comparisons test, p<0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24732703), licensed under a CC-BY license. Not internally tested by R&D Systems.