Mouse G-CSFR/CD114 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6039A

R&D Systems, part of Bio-Techne
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FAB6039A
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AF6039
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunohistochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Sheep IgG

View all G-CSFR/CD114 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse G‑CSF R/CD114
Cys26-Asp626
Accession # P40223

Specificity

Detects mouse G‑CSF R/CD114 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GM-CSF R alpha and recombinant mouse GM-CSF R alpha is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse G-CSFR/CD114 APC-conjugated Antibody

Detection of G‑CSF R/CD114 in Mouse Bone Marrow by Flow Cytometry.

Mouse bone marrow cells were stained with Rat Anti-Mouse Gr-1/Ly-6G PE-conjugated Monoclonal Antibody (FAB1037P) and either (A) Sheep Anti-Mouse G-CSF R/CD114 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6039A) or (B) Normal Sheep IgG Allophycocyanin Control (IC016A). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Mouse G-CSFR/CD114 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse bone marrow cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: G-CSFR/CD114

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic and granulocyte lineage (1). G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Cell activation by G‑CSF is mediated by granulocyte colony stimulating factor receptor alpha (G-CSF R; also CD114), a 95‑105 kDa type I transmembrane protein and member of the cytokine receptor superfamily, type I cytokine receptor family, and type 2 subfamily of receptor proteins. Mouse G‑CSF R is synthesized as an 837 amino acid (aa) precursor that contains a 25 aa signal sequence, a 601 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 187 aa cytoplasmic tail. The ECD contains one Ig-like C2-type domain, five fibronectin type-III domains, and 11 potential sites for N‑linked glycosylation. Within the ECD there is also a WSXWS motif (aa 319‑323) that is necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding (2). Also, within the cytoplasmic domain there is a Box 1 motif which is required for JAK interaction and/or activation (1). Mouse G‑CSF R shares 63% aa sequence identity with human G‑CSF R. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta (1). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia (1). These mutations typically lead to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophilic precursors in the bone marrow and neutropenia in peripheral blood (3). Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases (2). Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3 and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (4).

References

  1. Ward, A.C. (2007) Front. Biosci. 12:608.   
  2. Layton, J.E. and N.E. Hall (2006) Front. Biosci. 11:3181.
  3. Mitsui, T. et al. (2003) Blood 101:2990.
  4. Nicola, N.A. in Cytokine Reference, 2001, Oppenheim, J.J. and M. Feldmann, eds. Academic Press p.1935.
    5.  

Long Name

Granulocyte Colony Stimulating Factor Receptor

Alternate Names

CD114, CSF3R, Csfgr, G-CSF R, GCSFR

Entrez Gene IDs

1441 (Human); 12986 (Mouse)

Gene Symbol

CSF3R

UniProt

P40223

Additional G-CSFR/CD114 Products

Product Documents for Mouse G-CSFR/CD114 APC-conjugated Antibody

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Product Specific Notices for Mouse G-CSFR/CD114 APC-conjugated Antibody

For research use only

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