Detects mouse Glut2. Stains mouse Glut2-transfected NS0 cells but not NS0 control transfectants. It also detects Glut2 on mouse insulinoma betaTC-6 cells (2).

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse Glut2 Biotinylated Antibody

Glut2 in betaTC‑6 Mouse Cell Line.

Glut2 was detected in immersion fixed betaTC-6 mouse beta cell insulinoma cell line using Rat Anti-Mouse Glut2 Biotinylated Monoclonal Antibody (Catalog # BAM1440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse Glut2 Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

2.5 µg/10⁶ cells
Sample: betaTC-6 mouse beta cell insulinoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed betaTC-6 mouse beta cell insulinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glut2

Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells. Glut2 facilitates either glucose uptake or efflux from cells depending on the nutritional state (1).

References

Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
Poitout, V. et al. (1995) Diabetes 44:306.

Long Name

Glucose Transporter Type 2

Alternate Names

SLC2A2

Entrez Gene IDs

6514 (Human); 20526 (Mouse)

Gene Symbol

SLC2A2

UniProt

P14246

Additional Glut2 Products

Product Documents for Mouse Glut2 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Glut2 Biotinylated Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Glut2 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM1440

Key Product Details

Species Reactivity

Validated:

Cited:

Applications