Mouse gp130 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4681A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 125623
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse gp130
Gln23-Glu617 (predicted)
Accession # Q6PDI9
Gln23-Glu617 (predicted)
Accession # Q6PDI9
Specificity
Detects mouse gp130 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human gp130 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse gp130 APC-conjugated Antibody
Detection of gp130 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse gp130 APC-conjugated Monoclonal Antibody (Catalog # FAB4681A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse gp130 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Reviewed Applications
Read 1 review rated 5 using FAB4681A in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: gp130
Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.
Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.
References
- Narazaki, M. et al. (1993) Blood 82:1120.
- Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Long Name
Glycoprotein 130
Alternate Names
CD130, IL6ST
Gene Symbol
IL6ST
UniProt
Additional gp130 Products
Product Documents for Mouse gp130 APC-conjugated Antibody
Product Specific Notices for Mouse gp130 APC-conjugated Antibody
For research use only
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