Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rabbit Anti-Mouse IL-17F Monoclonal Antibody (Catalog # MAB20571) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).

Applications for Mouse IL-17F Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse splenocytes stimulated to induce Th17 cells

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17F

IL-17F, also called ML-1 or IL-24, is one of six IL-17 family members. All are homodimeric glycoproteins with conserved cysteines that form a cystine knot structure. IL-17F is a 44 kDa dimer expressed by activated monocytes that acts in a stimulation loop with activated CD4+T cells to produce IL-2, TGFb and MCP-1. Like IL-17A, it recruits neutrophils to airways during allergic reactions. IL-17F has a long form and a short form (predominant), which have alternate start sites but similar function. Mouse IL-17F is 58% aa identical to human IL-17F.

Long Name

Interleukin 17F

Alternate Names

IL17F

Entrez Gene IDs

112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)

Gene Symbol

IL17F

UniProt

Q7TNI7

Additional IL-17F Products

Product Documents for Mouse IL-17F Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse IL-17F Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse IL-17F Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB20571

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IL-17F Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse IL-17F Antibody

Detection of IL-17F in Mouse Splenocytes Stimulated to Induce Th17 cells by Flow Cytometry.