Mouse IL-1 beta/IL-1F2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB401R

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB401R-01M
MAB401R-100
MAB401R-500
MAB401R-SP
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry, Neutralization

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG1 Clone # 30311R

View all IL-1 beta/IL-1F2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-1 beta/IL‑1F2
Val118-Ser269
Accession # P10749

Specificity

Detects mouse IL-1 beta/IL‑1F2 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IL-1 beta/IL-1F2 Antibody

IL-1 beta/IL-1F2 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

IL‑1 beta/IL‑1F2 in Mouse Splenocytes.

IL-1 beta/IL-1F2 was detected in immersion fixed mouse splenocytes treated with LPS using Recombinant Rat Anti-Mouse IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB401R) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutralization by Mouse IL-1 beta/IL-1F2 Antibody.

Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutralization by Mouse IL-1 beta/IL-1F2 Antibody.

Recombinant Mouse IL-1 beta/IL-1F2 (Catalog # 401-ML) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-1 beta/IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of the Recombinant Rat Anti-Mouse IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB401R). The ND50 is typically 1-5 µg/mL.

Applications for Mouse IL-1 beta/IL-1F2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed mouse splenocytes treated with LPS

Neutralization

Measured by its ability to neutralize IL-1 beta/IL-1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 1-5 μg/mL in the presence of 50 pg/mL Recombinant Mouse IL-1 beta/IL-1F2.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

P10749

Additional IL-1 beta/IL-1F2 Products

Product Documents for Mouse IL-1 beta/IL-1F2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse IL-1 beta/IL-1F2 Antibody

For research use only

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