Mouse IL-1 beta/IL-1F2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AB-401-NA

R&D Systems, part of Bio-Techne
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AB-401-NA
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Neutralization, Simple Western, Western Blot

Cited:

Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IL-1 beta/IL-1F2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-1 beta/IL-1F2
Val118-Ser269
Accession # P10749

Specificity

Detects mouse IL-1 beta/IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant cotton rat IL-1 beta and recombinant rhesus monkey IL-1 beta is observed, approximately 15% cross-reactivity with recombinant rat IL-1 beta and recombinant rabbit IL-1 beta is observed, and less than 10% cross-reactivity with recombinant canine IL-1 beta, recombinant equine IL-1 beta, recombinant guinea pig IL-1 beta, recombinant porcine IL-1 beta, recombinant feline IL-1 beta, and recombinant human IL-1 beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IL-1 beta/IL-1F2 Antibody

Cell Proliferation Induced by IL‑1 beta/IL‑1F2 and Neutral-ization by Mouse IL‑1 beta/IL‑1F2 Antibody.

Cell Proliferation Induced by IL‑1 beta/IL‑1F2 and Neutral-ization by Mouse IL‑1 beta/IL‑1F2 Antibody.

Recombinant Mouse IL-1 beta/IL-1F2 (401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta/IL-1F2 (10 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta/IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA). The ND50 is typically 2-12 µg/mL.
Detection of Human and Mouse IL-1 beta/IL-1F2 antibody by Western Blot.

Detection of Human and Mouse IL‑1 beta/IL‑1F2 by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 4 hours and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IL-1 beta/IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-1 beta/IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse IL-1 beta/IL-1F2 antibody by Simple WesternTM.

Detection of Mouse IL‑1 beta/IL‑1F2 by Simple WesternTM.

Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL‑1 beta/IL‑1F2 at approximately 40 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse IL‑1 beta/IL‑1F2 Polyclonal Antibody (Catalog # AB-401-NA) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse IL-1 beta/IL-1F2 Antibody

Application
Recommended Usage

Simple Western

50 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line treated with LPS

Western Blot

1 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line treated with PMA and LPS and RAW 264.7 mouse monocyte/macrophage cell line treated with LPS

Neutralization

Measured by its ability to neutralize IL‑1 beta/IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line [Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272]. The Neutralization Dose (ND50) is typically 2-12 µg/mL in the presence of 10 pg/mL Recombinant Mouse IL‑1 beta/IL‑1F2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

P10749

Additional IL-1 beta/IL-1F2 Products

Product Documents for Mouse IL-1 beta/IL-1F2 Antibody

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Certificate of Analysis

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Product Specific Notices for Mouse IL-1 beta/IL-1F2 Antibody

For research use only

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