Mouse IL-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB702
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Adoptive Transfer, ELISA Development, ELISpot Development, Luminex Development, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # JES6-1A12
Product Specifications
Immunogen
E. coli-derived recombinant mouse IL-2
Specificity
Detects mouse IL-2 in ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse IL-2 Antibody
Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody.
Recombinant Mouse IL-2 (Catalog # 402-ML) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-2 Monoclonal Antibody (Catalog # MAB702). The ND50 is typically 0.125-0.75 µg/mL.Applications for Mouse IL-2 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND50) is typically 0.125-0.75 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑2.
Mouse IL-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2
Long Name
Interleukin 2
Alternate Names
Aldesleukin, IL2, Proleukin, TCGF
Entrez Gene IDs
Gene Symbol
IL2
Additional IL-2 Products
Product Documents for Mouse IL-2 Antibody
Product Specific Notices for Mouse IL-2 Antibody
For research use only
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