Mouse IL-5 R alpha/CD125 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF553
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑5 R alpha/CD125
Asp18-His339
Accession # P21183
Asp18-His339
Accession # P21183
Specificity
Detects mouse IL‑5 R alpha/CD125 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) IL-5 R alpha is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-4 R, rhIL-9 R, rhIL-13 R alpha1, and rmIL-13 R alpha2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse IL-5 R alpha/CD125 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IL‑5 R alpha/CD125 Fc Chimera (Catalog # 553-MR)
Sample: Recombinant Mouse IL‑5 R alpha/CD125 Fc Chimera (Catalog # 553-MR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5 R alpha/CD125
References
- Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
- Rothenberg, M.E. and S.P. Hogan (2005) Annu. Rev. Immunol. 24:147.
- Elsas, X.P. and M.I.G. Elsas (2007) Curr. Med. Chem. 14:1925.
- Takaki, S. et al. (1990) EMBO J. 9:4367.
- Cameron, L. et al. (2000) J. Immunol. 164:1538.
- Tavernier, J. et al. (1991) Cell 66:1175.
- Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
- Tavernier, J. et al. (2000) Blood 95:1600.
- Clutterbuck, E.J. et al. (1989) Blood 73:1504.
- Lopez, A.F. et al. (1988) J. Exp. Med. 167:219.
- Denburg, J.A. et al. (1991) Blood 77:1462.
- Hasbold, J. et al. (2004) Nat. Immunol. 5:55.
- Gregory, B. et al. (2003) J. Immunol. 170:5359.
- Liu, L.Y. et al. (2002) J. Immunol. 169:6459.
Long Name
Interleukin 5 Receptor alpha
Alternate Names
CD125, IL-5Ra, IL5R alpha, IL5RA
Gene Symbol
IL5RA
UniProt
Additional IL-5 R alpha/CD125 Products
Product Documents for Mouse IL-5 R alpha/CD125 Biotinylated Antibody
Product Specific Notices for Mouse IL-5 R alpha/CD125 Biotinylated Antibody
For research use only
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