Mouse IL-5 R alpha/CD125 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF553

R&D Systems, part of Bio-Techne
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AF553
AF553-SP
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Biotin
BAF553
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IL-5 R alpha/CD125 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑5 R alpha/CD125
Asp18-His339
Accession # P21183

Specificity

Detects mouse IL‑5 R alpha/CD125 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) IL‑5 R alpha is observed and less than 1% cross‑reactivity with recombinant mouse (rm) IL-4 R, rhIL-9 R, rhIL-13 R alpha1, and rhIL-13 R alpha2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse IL-5 R alpha/CD125 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL‑5 R alpha/CD125 Fc Chimera (Catalog # 553-MR)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5 R alpha/CD125

Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology (1‑3). Mature mouse IL‑5 R alpha consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 22 aa transmembrane segment, and a 54 aa cytoplasmic domain (4). Within the ECD, mouse IL‑5 R alpha shares 71% and 86% aa sequence identity with human and rat IL‑5 R alpha, respectively. Alternate splicing of mouse IL‑5 R alpha generates soluble secreted forms which function as IL‑5 antagonists (4, 5). The high affinity receptor for IL‑5 is a complex that consists of the ligand binding IL‑5 R alpha and the transmembrane common  beta chain ( betac/CD131) which is shared with the receptor complexes for IL‑3 and GM‑CSF (6). IL‑5 R alpha binds IL‑5 at low affinity and then associates with preformed betac oligomers to form the signaling‑competent receptor complex (7). IL‑5 stimulation of CD34+ hematopoietic progenitor cells induces the up‑regulation of transmembrane IL‑5 R alpha followed by eosinophilic differentiation and activation (8‑10). IL‑5 R alpha also promotes the differentiation of basophils and B cells (11, 12). Exposure of mature eosinophils to IL‑5 attenuates their IL‑5 responsiveness by inducing the down‑regulation of surface IL‑5 R alpha and increased production of soluble IL‑5 R alpha (13, 14). Elevated production of IL‑5 at sites of allergic inflammation induces eosinophilia and exacerbation of immune cell infiltration, tissue damage, and remodeling (2, 3).

References

  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Rothenberg, M.E. and S.P. Hogan (2005) Annu. Rev. Immunol. 24:147.
  3. Elsas, X.P. and M.I.G. Elsas (2007) Curr. Med. Chem. 14:1925.
  4. Takaki, S. et al. (1990) EMBO J. 9:4367.
  5. Cameron, L. et al. (2000) J. Immunol. 164:1538.
  6. Tavernier, J. et al. (1991) Cell 66:1175.
  7. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
  8. Tavernier, J. et al. (2000) Blood 95:1600.
  9. Clutterbuck, E.J. et al. (1989) Blood 73:1504.
  10. Lopez, A.F. et al. (1988) J. Exp. Med. 167:219.
  11. Denburg, J.A. et al. (1991) Blood 77:1462.
  12. Hasbold, J. et al. (2004) Nat. Immunol. 5:55.
  13. Gregory, B. et al. (2003) J. Immunol. 170:5359.
  14. Liu, L.Y. et al. (2002) J. Immunol. 169:6459.

Long Name

Interleukin 5 Receptor alpha

Alternate Names

CD125, IL-5Ra, IL5R alpha, IL5RA

Entrez Gene IDs

3568 (Human); 16192 (Mouse)

Gene Symbol

IL5RA

UniProt

P21183

Additional IL-5 R alpha/CD125 Products

Product Documents for Mouse IL-5 R alpha/CD125 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse IL-5 R alpha/CD125 Antibody

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