Mouse Integrin beta7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB30601
Key Product Details
Species Reactivity
Mouse
Applications
Adhesion Activation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 332408
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta7
Glu20-His722
Accession # P26011
Glu20-His722
Accession # P26011
Specificity
Detects mouse Integrin beta7 extracellular domain in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Mouse Integrin beta7 Antibody
Application
Recommended Usage
Adhesion Activation
The adhesion of TK-1 mouse T cell lymphoma cells (5 x 104 cells/well, treated with 1 mM MnCl2) to immobilized Recombinant Mouse MAdCAM-1 Fc Chimera (Catalog # 993-MC, 1 µg/well, 100 µL/well) was maximally enhanced (2‑2.5 fold) by 10 µg/mL of the antibody.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 7
Integrin beta7 associates with the integrin alpha4 or alphaE subunits to form heterodimers to interact with fibronectin, VCAM, MAdCAM or E-Cadherin. It is highly expressed in leukocytes and is involved in localization of leukocytes at mucosal sites.
Alternate Names
ITGB7
Gene Symbol
ITGB7
UniProt
Additional Integrin beta 7 Products
Product Documents for Mouse Integrin beta7 Antibody
Product Specific Notices for Mouse Integrin beta7 Antibody
For research use only
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