Mouse LAG-3 Antibody

Detection of Mouse LAG-3 by Western Blot.

Western blot shows lysates of mouse liver tissue, mouse heart tissue, EL4.IL-2 mouse lymphoblast cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse LAG-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3328) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for LAG-3 at approximately 54 kDa and 75 kDa in mouse liver and mouse heart tissue and 70-80 kDa in EL4.IL-2 and CTLL-2 cell lines (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

LAG-3 in Mouse Spleen.

LAG-3 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Goat Anti-Mouse LAG-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3328) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

LAG-3 in Human Spleen.

LAG-3 was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Mouse LAG-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3328) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.