Mouse Laminin alpha 4 Biotinylated Antibody

Name: Mouse Laminin alpha 4 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF3837

R&D Systems, part of Bio-Techne | Catalog # BAF3837

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

AF3837

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Laminin alpha 4 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Laminin alpha4
Gln826-Ala1816
Accession # P97927

Specificity

Detects mouse Laminin alpha4 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Laminin alpha 4 Biotinylated Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E11.5)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Laminin alpha4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 4

Laminins are heterotrimeric glycoproteins and are major components of the basement membrane. Each laminin is comprised of a single alpha, beta, and gamma chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule. Mature Laminin alpha4 is a subunit of Laminin 411, Laminin 421, and Laminin 423. Mature Laminin alpha4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C‑terminal tandem Laminin G-like domains. The G-like domains contain binding sites for integrin, heparin, and dystroglycan. Within the region used as immunogen, mouse Laminin alpha4 shows 91% and 97% aa sequence identity with human and rat Laminin alpha4, respectively.

Alternate Names

LAMA4

Entrez Gene IDs

3910 (Human); 16775 (Mouse); 309816 (Rat)

Gene Symbol