Mouse Laminin alpha4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3837
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Rat, Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Laminin alpha4
Gln826-Ala1816
Accession # P97927
Gln826-Ala1816
Accession # P97927
Specificity
Detects mouse Laminin alpha4 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Laminin alpha4 Antibody
Laminin alpha4 in Mouse Embryo.
Laminin a4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Laminin a4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3837) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to membranes in blood vessels in CNS. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Laminin alpha4 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Laminin alpha4
Sample: Recombinant Mouse Laminin alpha4
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 3.5 using AF3837 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin alpha 4
Alternate Names
LAMA4
Gene Symbol
LAMA4
UniProt
Additional Laminin alpha 4 Products
Product Documents for Mouse Laminin alpha4 Antibody
Product Specific Notices for Mouse Laminin alpha4 Antibody
For research use only
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