Mouse Limitin/IFN-zeta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5971
Key Product Details
Species Reactivity
Mouse
Applications
ELISA Capture (Matched Antibody Pair), Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 183707
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Limitin/IFN-zeta
Leu22-Arg182
Accession # Q9R1T0
Leu22-Arg182
Accession # Q9R1T0
Specificity
Detects mouse Limitin/IFN-zeta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Mouse Limitin/IFN-zeta Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize Limitin inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin/IFN-zeta (Catalog # 1535-LM).
Mouse Limitin Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Limitin/IFN-zeta
Limitin, also known as IFN-zeta, is a 20 kDa glycoprotein that is secreted by T cells and bronchial and salivary duct epithelial cells. It inhibits B-lineage cell development but has little effect on myelopoiesis or erythropoiesis. Limitin binds to IFN-alpha / beta receptors and has potent antiviral activity. Mouse Limitin shares 32%, 26%, and 30% amino acid sequence identity with mouse IFN-alpha, mouse IFN-beta, and human IFN-omega, respectively.
Long Name
Interferon zeta
Alternate Names
IFN-z, IFNzeta
Entrez Gene IDs
319146 (Mouse)
Gene Symbol
IFNZ
UniProt
Additional Limitin/IFN-zeta Products
Product Documents for Mouse Limitin/IFN-zeta Antibody
Product Specific Notices for Mouse Limitin/IFN-zeta Antibody
For research use only
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