Mouse Limitin/IFN- zeta Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF597
Key Product Details
Species Reactivity
Mouse
Applications
ELISA Detection (Matched Antibody Pair), Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-expressed human LAT
aa 30-113/His
Accession # Q9R1T0
aa 30-113/His
Accession # Q9R1T0
Specificity
Detects Limitin/IFN-zeta in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) IFN-gamma and rmIFN-alpha / beta R1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Limitin/IFN- zeta Biotinylated Antibody
Detection of Limitin/IFN- zeta in Mouse Thymus.
Limitin/IFN- zeta was detected in immersion fixed paraffin-embedded sections of mouse thymus using Goat Anti-Mouse Limitin/IFN- zeta Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF597) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and B cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Mouse Limitin/IFN- zeta Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus
Sample: Perfusion fixed frozen sections of mouse thymus
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Limitin/IFN-zeta (Catalog # 1535-LM)
Sample: Recombinant Mouse Limitin/IFN-zeta (Catalog # 1535-LM)
Mouse Limitin Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Limitin/IFN-zeta
Limitin, also known as IFN-zeta, is a 20 kDa glycoprotein that is secreted by T cells and bronchial and salivary duct epithelial cells. It inhibits B-lineage cell development but has little effect on myelopoiesis or erythropoiesis. Limitin binds to IFN-alpha / beta receptors and has potent antiviral activity. Mouse Limitin shares 32%, 26%, and 30% amino acid sequence identity with mouse IFN-alpha, mouse IFN-beta, and human IFN-omega, respectively.
Long Name
Interferon zeta
Alternate Names
IFN-z, IFNzeta
Entrez Gene IDs
319146 (Mouse)
Gene Symbol
IFNZ
UniProt
Additional Limitin/IFN-zeta Products
Product Documents for Mouse Limitin/IFN- zeta Biotinylated Antibody
Product Specific Notices for Mouse Limitin/IFN- zeta Biotinylated Antibody
For research use only
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