Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Monoclonal Antibody (Catalog # BAM1037) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse splenocytes

Immunohistochemistry

Nitta, H. et al. (1997) Cell Vision 4:73.

Immunoprecipitation

Conlan, J.W. and R.J. North (1994) J. Exp. Med. 179:259.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ly-6G/Ly-6C (Gr-1)

Gr-1, also called Ly-6G, is a myeloid differentiation antigen that is a member of the Ly-6 family. The RB6‑8C5 antibody recognizes Gr-1/Ly-6G, and also reacts weakly with Ly-6C-transfected EL4 cells (1, 2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References

Spangrude, G.J. et al. (1988) Science 241:58
Fleming, T.J. et al. (1993) J. Immunol. 151:2399
Lewinsohn, D.M. et al. (1987) J. Immunol. 147:22
Lagasse, E. and I. L. Weissman (1996) J. Immunol. Methods 197:139

Long Name

A Myeloid Differentiation Antigen

Alternate Names

Ly-6C, Ly-6G, Ly6G

Entrez Gene IDs

546644 (Mouse)

Gene Symbol

LY6G

Additional Ly-6G/Ly-6C (Gr-1) Products

Product Documents for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM1037

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Ly-6G/Ly-6C (Gr-1) Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

Ly-6G/Ly-6C (Gr-1) in Mouse Spleen.