Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM1037
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Cited:
Immunohistochemistry-Paraffin
Label
Biotin
Antibody Source
Monoclonal Rat IgG2B Clone # RB6-8C5
Product Specifications
Immunogen
The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
Specificity
Detects mouse Ly-6G/Ly-6C (Gr-1). Weak cross-reactivity with Ly-6C is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody
Ly-6G/Ly-6C (Gr-1) in Mouse Spleen.
Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Monoclonal Antibody (Catalog # BAM1037) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Immunohistochemistry
Nitta, H. et al. (1997) Cell Vision 4:73.
Immunoprecipitation
Conlan, J.W. and R.J. North (1994) J. Exp. Med. 179:259.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ly-6G/Ly-6C (Gr-1)
References
- Spangrude, G.J. et al. (1988) Science 241:58
- Fleming, T.J. et al. (1993) J. Immunol. 151:2399
- Lewinsohn, D.M. et al. (1987) J. Immunol. 147:22
- Lagasse, E. and I. L. Weissman (1996) J. Immunol. Methods 197:139
Long Name
A Myeloid Differentiation Antigen
Alternate Names
Ly-6C, Ly-6G, Ly6G
Entrez Gene IDs
546644 (Mouse)
Gene Symbol
LY6G
Additional Ly-6G/Ly-6C (Gr-1) Products
Product Documents for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody
Product Specific Notices for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody
For research use only
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