Mouse MEPE/OF45 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6150
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MEPE/OF45
Ala17-Asp433
Accession # AAK70342
Ala17-Asp433
Accession # AAK70342
Specificity
Detects mouse MEPE/OF45 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human MEPE/OF45 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse MEPE/OF45 Antibody
Detection of Mouse MEPE/OF45 by Western Blot.
Western blot shows lysates of ST-2 mouse bone marrow-derived stromal cell line and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse MEPE/OF45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6150) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for MEPE/OF45 at approximately 58 and 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse MEPE/OF45 Antibody
Application
Recommended Usage
Western Blot
1.0 µg/mL
Sample: ST‑2 mouse bone marrow-derived stromal cell line and mouse embryo tissue
Sample: ST‑2 mouse bone marrow-derived stromal cell line and mouse embryo tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MEPE/OF45
MEPE (matrix extracellular phosphoglycoprotein; also OF45) is a 56‑58 kDa member of the SIBLING protein family. It is expressed by osteocytes, osteoblasts, odontoblasts and chondrocytes. MEPE is found both intra- and extracellularly. Within the cell, it binds to CHK1. This protects CHK1 from degradation, and the cell from DNA damage. Extracellularly, it binds to Phex, which protects it from cathepsin B proteolysis. Undegraded, it promotes bone vascularization and blocks bone resorption. Following cleavage after Arg410 (in mouse), a 2 kDa ASARM fragment is generated that, when phosphorylated, blocks mineralization. Mature mouse MEPE is 417 amino acids (aa) in length. It contains an RGD motif and ASARM domain (aa 416‑433). In addition to its a 57 kDa form, 70 kDa, 42‑45 kDa and 30‑35 kDa forms of mouse MEPE have been reported. Whether they are cleavage forms or products of glycosylation is unclear. Over aa 147‑433, mouse MEPE shares 77% and 57% aa identity with rat and human MEPE, respectively.
Long Name
Matrix Extracellular Phosphoglycoprotein with ASARM Motif
Alternate Names
OF45, Osteoregulin
Gene Symbol
MEPE
UniProt
Additional MEPE/OF45 Products
Product Documents for Mouse MEPE/OF45 Antibody
Product Specific Notices for Mouse MEPE/OF45 Antibody
For research use only
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