Neuro-2A mouse neuroblastoma cell line was stained with Rat Anti-Mouse Meteorin-like/METRNL APC-conjugated Monoclonal Antibody (Catalog # IC6679A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Mouse Meteorin-like/METRNL APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Neuro‑2A mouse neuroblastoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Meteorin-like/METRNL

Meteorin-like protein (METRNL) is a 311 amino acid (aa) protein that shows 45% aa homology with Meteorin (in mouse). Meteorin itself is a secreted 30 kDa protein that is expressed in glial and neuronal cells and their precursors during development and in the adult (1‑3). In the adult Meteorin is primarily expressed by astrocytes (3). Meteorin promotes neurite outgrowth, glial cell differentiation, and neuronal survival following excitotoxic injury (2‑5). It also inhibits sprouting angiogenesis and promotes blood vessel maturation (1). Mouse Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide and is selectively expressed in the otic vesicle during inner ear development (6, 7). Mature mouse Meteorin‑like protein shares 79% and 98% aa sequence identity with human and rat Meteorin‑like protein, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide. R&D Systems in‑house testing indicates that Meteorin‑like protein enhances the outgrowth of neurites from cultured mouse cortical neurons. The promoter region of the METRNL gene contains a recognition site for the Pax2, Pax5, and Pax8 transcription factors, and Pax2 binds specifically to this region (7). METRNL is the only gene found within the terminus of the human chromosome 17 q‑arm which can be deleted in the rare Ring 17 syndrome (8).

References

Park, J.A. et al. (2008) Glia 56:247.
Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104.
Lee, H.S. et al. (2010) J. Cell Sci. 123:1959.
Nishino, J. et al. (2004) EMBO J. 23:1998.
Jorgensen, J.R. et al. (2011) Neurobiol. Dis. 41:160.
Accession # Q8VE43.
Ramialison, M. et al. (2008) Genome Biol. 9:R145.
Surace, C. et al. (2009) Clin. Genet. 76:256.

Alternate Names

Meteorin like, METRNL

Entrez Gene IDs

284207 (Human); 210029 (Mouse); 316842 (Rat)

Gene Symbol

METRNL

UniProt

Q8VE43

Additional Meteorin-like/METRNL Products

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SDS

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Product Specific Notices for Mouse Meteorin-like/METRNL APC-conjugated Antibody

For research use only

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Flow Cytometry

Mouse Meteorin-like/METRNL APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC6679A

Key Product Details

Species Reactivity

Applications

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Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Meteorin-like/METRNL APC-conjugated Antibody

Detection of Meteorin-like/METRNL in Neuro-2A Mouse Cell Line by Flow Cytometry.