Mouse MHC class II (I-A/I-E) APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB6118A
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # M5/114.15.2
Product Specifications
Immunogen
Activated C57BL/6 mouse spleen cells
Specificity
Detects mouse MHC class II (I‑A/I‑E) in flow cytometry.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse MHC class II (I-A/I-E) APC-conjugated Antibody
Detection of MHC class II (I‑A/I‑E) in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4841F) and either (A) Rat Anti-Mouse MHC class II (I-A/I-E) APC-conjugated Monoclonal Antibody (Catalog # FAB6118A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse MHC class II (I-A/I-E) APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: MHC class II (I-A/I-E)
References
- Bhattacharya, A. et al. (1981) J. Immunol. 127:2448.
- Watts, C. et al. (1997) Ann. Rev. Immunol. 15:821.
Long Name
Major Histocompatibility Complex Class II IA and IE
Alternate Names
H2-Ea, MHC class II IA/IE
Entrez Gene IDs
14968 (Mouse)
Gene Symbol
H2-EA-PS
Additional MHC class II (I-A/I-E) Products
Product Documents for Mouse MHC class II (I-A/I-E) APC-conjugated Antibody
Product Specific Notices for Mouse MHC class II (I-A/I-E) APC-conjugated Antibody
For research use only
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