Mouse MINA Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7266
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse MINA
Pro2-Gly192
Accession # Q8CD15
Pro2-Gly192
Accession # Q8CD15
Specificity
Detects mouse MINA in direct ELISAs and Western blots. In direct ELISAs, 80% cross-reactivity with recombinant human (rh) MINA is observed and 40% cross-reactivity with rhMINA is observed in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse MINA Antibody
Detection of Mouse MINA by Western Blot.
Western blot shows lysates of P19 mouse embryonal carcinoma cell line and betaTC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse MINA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7266) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MINA at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse MINA Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: P19 mouse embryonal carcinoma cell line and betaTC‑6 mouse beta cell insulinoma cell line
Sample: P19 mouse embryonal carcinoma cell line and betaTC‑6 mouse beta cell insulinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MINA
MINA (myc-induced nuclear antigen; also Mina53) is a 52-54 kDa member of both the MINA53/NO66 and Jumonji C family of proteins. It expression is associated with proliferating cells, and it has been found in cytoplasm, nucleus and nucleoli. MINA appears to be induced by c-myc, and synthesized by spermatogonia, occasional squamous epithelium, naïve T cells and select cancer cells. When expressed, MINA is reported to regulate expression of genes such as HGF, EGF-R and IL-4. It may exert its regulatory activity through an intrinsic demethylase function. Mouse MINA is 465 amino acids (aa) in length. It possesses one cupin (or enzyme-associated) region (aa 51-363) that contains a JmjC domain (aa 139-271). There are two potential isoform variants that contain either a 12 aa substitution for aa 145-465, or a 15 aa substitution for aa 228-465. Over aa 2-192, mouse MINA shares 92% and 82% aa sequence identity with rat and human MINA, respectively.
Long Name
Myc-induced Nuclear Antigen
Alternate Names
MDIG, Mina53, NO52
Gene Symbol
RIOX2
UniProt
Additional MINA Products
Product Documents for Mouse MINA Antibody
Product Specific Notices for Mouse MINA Antibody
For research use only
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