Mouse splenocytes treated with 100 ng/mL Recombinant Mouse IL-2 (Catalog # 402-ML) overnight were stained with Rat Anti-Mouse NKp46/NCR1 APC-conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rabbit Anti-Mouse NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB8888) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Applications for Mouse NFIL3/E4BP4 Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse splenocytes treated with Recombinant Mouse IL‑2 (Catalog # 402-ML) were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFIL3/E4BP4

NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor of the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in T and pro-B lymphocytes and cardiomyocytes, binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs; 1,2). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.

Long Name

Nuclear Factor, Interleukin 3 Regulated

Alternate Names

E4BP4, IL3BP1, NF-IL3A

Entrez Gene IDs

4783 (Human); 18030 (Mouse); 114519 (Rat)

Gene Symbol

NFIL3

UniProt

O08750

Additional NFIL3/E4BP4 Products

Product Documents for Mouse NFIL3/E4BP4 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Mouse NFIL3/E4BP4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse NFIL3/E4BP4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8888

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse NFIL3/E4BP4 Antibody

Detection of NFIL3/E4BP4 in Mouse Splenocytes by Flow Cytometry.