HEK293 Human embryonic kidney cell line transfected with either (A) Mouse NKG2A/CD159a, or (B) Mouse NKG2C/CD159c and eGFP was stained with Rabbit Anti-Mouse NKG2A/CD159a Monoclonal Antibody (Catalog # MAB68671) followed by APC-conjugated anti-Rabbit IgG secondary antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse NKG2A/CD159a Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Mouse NKG2A/CD159a and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2A/CD159a

NKG2A, also known as CD159a, is a 244 amino acid (aa), ~38 kDa type II transmembrane glycoprotein with a cytoplasmic ITIM motif and a single extracellular lectin-like domain. Within the extracellular domain (aa 94-244), mouse NKG2A shares 71% and 41% aa sequence identity with rat and human NKG2A, respectively. An isoform lacking aa 98-114 has been described. NKG2A associates with CD94 and is coexpressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its murine ligand, Qa-1b (or HLA-E in humans) on antigen presenting cells.

Long Name

Natural Killer G2A

Alternate Names

CD159a, Klrc1, NKG2

Entrez Gene IDs

3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)

Gene Symbol

KLRC1

UniProt

Q9Z202

Additional NKG2A/CD159a Products

Product Documents for Mouse NKG2A/CD159a Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse NKG2A/CD159a Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse NKG2A/CD159a Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB68671

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all NKG2A/CD159a Antibodies »

Product Summary for Mouse NKG2A/CD159a Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse NKG2A/CD159a Antibody

Detection of NKG2A/CD159a in HEK293 Human Cell Line Transfected with Mouse NKG2A/CD159a and eGFP by Flow Cytometry.