HEK293 human embryonic kidney cell line transfected with either (A) mouse NKG2A or (B) irrelevant protein, and eGFP, was stained with Rat anti-mouse NKG2A monoclonal antibody (Catalog # MAB6867) followed by Allophycocyanin-conjugated anti-Rat IgG Secondary Antibody (F0113). Quadrant markers were set based on control antibody staining (MAB0061, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Detection of NKG2A/CD159a in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse NKG2A/CD159a Monoclonal Antibody (Catalog # MAB6867) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113) and Rat Anti-Mouse Integrin a2/CD49b PE-conjugated Monoclonal Antibody (Catalog # FAB1740P). Quadrant markers were set based on control antibody staining (Catalog # MAB0061).

Applications for Mouse NKG2A/CD159a Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line transfected with Mouse NKG2A and eGFP and Mouse splenocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2A/CD159a

NKG2A, also known as CD159a, is a 244 amino acid (aa), ~38 kDa type II transmembrane glycoprotein with a cytoplasmic ITIM motif and a single extracellular lectin-like domain. Within the extracellular domain (aa 94-244), mouse NKG2A shares 71% and 41% aa sequence identity with rat and human NKG2A, respectively. An isoform lacking aa 98-114 has been described. NKG2A associates with CD94 and is coexpressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its murine ligand, Qa-1b (or HLA-E in humans) on antigen presenting cells.

Long Name

Natural Killer G2A

Alternate Names

CD159a, Klrc1, NKG2

Entrez Gene IDs

3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)

Gene Symbol

KLRC1

UniProt

Q9Z202

Additional NKG2A/CD159a Products

Product Documents for Mouse NKG2A/CD159a Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse NKG2A/CD159a Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse NKG2A/CD159a Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6867

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

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Product Summary for Mouse NKG2A/CD159a Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse NKG2A/CD159a Antibody

Detection of NKG2A in HEK293 Human Cell Line transfected with Mouse NKG2A and eGFP by Flow Cytometry