Mouse Patched 1/PTCH Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11487
Key Product Details
Species Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 413218
Product Specifications
Immunogen
E. coli-derived recombinant mouse Patched 1/PTCH
Glu108-Asp422
Accession # Q61115
Glu108-Asp422
Accession # Q61115
Specificity
Detects mouse Patched 1/PTCH in direct ELISAs
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Patched 1/PTCH Antibody
Detection of Patched 1/PTCH in Neuro2a.
Patched 1/PTCH was detected in fixed Neuro2A cells using Rat Anti-Mouse Patched 1/PTCH Monoclonal Antibody (Catalog # MAB11487) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the membrame. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Patched 1/PTCH in Mouse Kidney.
Patched 1/PTCH was detected in immersion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Mouse Patched 1/PTCH Monoclonal Antibody (Catalog # MAB11487) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Mouse Patched 1/PTCH Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: fixed Neuro2A cells
Sample: fixed Neuro2A cells
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse kidney
Sample: Immersion fixed paraffin-embedded sections of mouse kidney
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Patched 1/PTCH
Patched is a 170‑175 kDa member of the patched family of proteins. It is found on multiple cells types and forms a constitutive, inactivating complex with Smoothened. Upon SHH binding to Patched, Smoothened is released and initiates downstream signaling. Mouse Patched is a 12 transmembrane, 1434 amino acid (aa) glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for Patched has been reported in mice. However, it would appear likely that two or more splice forms exist, with variation occurring over the first 150 aa of the N-terminus. Over aa 108‑422, mouse Patched shares 97% and 99% aa sequence identity with human and rat Patched, respectively.
Alternate Names
BCNS, HPE7, mes, NBCCS, ptc1, PTCH1
Gene Symbol
PTCH1
UniProt
Additional Patched 1/PTCH Products
Product Documents for Mouse Patched 1/PTCH Antibody
Product Specific Notices for Mouse Patched 1/PTCH Antibody
For research use only
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