Mouse Rae-1 Pan Specific Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB17582
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, CyTOF-ready
Cited:
Western Blot, Flow Cytometry, Immunocytochemistry, Surface Plasmon Resonance
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 186107
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Rae-1 delta
Leu29-Ser227
Accession # Q9JI58
Leu29-Ser227
Accession # Q9JI58
Specificity
Detects mouse Rae‑1. It recognizes Rae-1 alpha, beta, delta, gamma and epsilon.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Rae-1 Pan Specific Antibody
Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific Monoclonal Antibody (Catalog # MAB17582, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.Detection of Rae-1 Pan Specific by Flow Cytometry
NK cell mediated non-self or induced-self recognition does not impact the expansion of BCR-ABL1+ cells.(A) The bar graph shows the mean absolute number (±SD) of B6 Dd (H-2bDd, CD45.1)-derived myeloid cells expressing BCR-ABL1 (GFP) in B6 (H-2b, CD45.2) recipients at day 8 after transplantation (MHC-I different, non-self recognition). (B) The expression of Rae-1 NKG2D ligands was determined on B6 (left) or BALB.B (right)-derived myeloid cells (CD11b+) expressing BCR-ABL1 on day 8 after transplantation into B6 mice (gray fill). Open histograms depict background staining on BM-derived CD11b+ cells of normal B6 mice. (C) The bar graph shows the mean absolute number (±SD) of BABL.B (H-2b, CD45.2)-derived myeloid cells expressing GFP (either control or BCR-ABL1) in B6 (H-2b, CD45.1) recipients at day 8 after transplantation (MHC-I matched, unrelated, induced-self recognition). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22132120), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse Rae-1 Pan Specific Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rae-1
Long Name
Retinoic Acid Early Transcript 1
Alternate Names
Rae1
Gene Symbol
RAE1
UniProt
Additional Rae-1 Products
Product Documents for Mouse Rae-1 Pan Specific Antibody
Product Specific Notices for Mouse Rae-1 Pan Specific Antibody
For research use only
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