RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific PE-conjugated Monoclonal Antibody (Catalog # FAB17582P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Rae-1 Pan Specific PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

0.25-1 µg/10⁶ cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Rae-1

Rae-1 alpha, beta, gamma, delta and epsilon comprise a family of closely related (88-95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Long Name

Retinoic Acid Early Transcript 1

Alternate Names

Rae1

Entrez Gene IDs

8480 (Human); 19368 (Mouse)

Gene Symbol

RAE1

UniProt

Q9JI58

Additional Rae-1 Products

Product Documents for Mouse Rae-1 Pan Specific PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Rae-1 Pan Specific PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse Rae-1 Pan Specific PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB17582P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Rae-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Rae-1 Pan Specific PE-conjugated Antibody

Detection of Rae-1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.