Mouse/Rat CD34 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6518
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Flow Ctyometry, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD34
Thr35-Thr287
Accession # Q64314
Thr35-Thr287
Accession # Q64314
Specificity
Detects mouse and rat CD34 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant porcine CD34 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse/Rat CD34 Antibody
Detection of Mouse and Rat CD34 by Western Blot.
Western blot shows lysates of mouse brain (cortex) tissue and rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6518) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD34 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of CD34 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Mouse CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6518, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).Applications for Mouse/Rat CD34 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line
Sample: bEnd.3 mouse endothelioma cell line
Western Blot
1 µg/mL
Sample: Mouse brain (cortex) tissue and rat testis tissue
Sample: Mouse brain (cortex) tissue and rat testis tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF6518 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD34
Mouse and rat CD34 is a 80 -100 kDa member of the CD34/podocalyxin family of molecules. It is a sialomucin type glycoprotein, and presents carbohydrate to selectins during cell migration. CD34 is found on mast cells, eosinophils, vascular endothelial cells, CD34+ stem cells and renal mesangial cells. Mature mouse CD34 is a 348 amino acid (aa) type I transmembrane protein (aa 35-382). It contains a 253 aa extracellular region (aa 35-287), and a 74 aa cytoplasmic domain that undergoes Tyr phosphorylation. Mouse and rat CD34 also is likely to undergo membrane cleavage that will generate a 54 kDa soluble form. Over aa 35-287, mouse CD34 shares 57% and 82% aa identity with human and rat Cd34, respectively.
Alternate Names
CD34, HPCA1
Gene Symbol
CD34
UniProt
Additional CD34 Products
Product Documents for Mouse/Rat CD34 Antibody
Product Specific Notices for Mouse/Rat CD34 Antibody
For research use only
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