Mouse/Rat Kynureninase Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7389
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Kynureninase
Gly267-Ser464
Accession # Q9CXF0
Gly267-Ser464
Accession # Q9CXF0
Specificity
Detects mouse and rat Kynureninase in Western blots. Detects recombinant mouse Kynureninase in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human Kynureninase is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse/Rat Kynureninase Antibody
Detection of Mouse and Rat Kynureninase by Western Blot.
Western blot shows lysates of mouse liver tissue and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse Kynureninase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7389) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kynureninase at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse/Rat Kynureninase Antibody
Application
Recommended Usage
Western Blot
0.25 µg/mL
Sample: Mouse liver tissue and rat liver tissue
Sample: Mouse liver tissue and rat liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kynureninase
Kynu (kyureninase (a hydrolase that acts on dog urine ) is a 52-55 kDa member of the kyureninase family of enzymes. It is a pyridoxal phosphate-dependent cytosolic hydrolase that occurs in a variety of cell types, including hepatocytes and macrophages. Kynu participates in the metabolism of tryptophan. Dietary Trp is either incorporated into protein, or metabolized into niacin, serotonin or NAD. Kynu catalyzes one of two steps that lead to the formation of a key downstream intermediate called 3-hydroxyanthranilic acid. Mouse Kynu is 464 amino acids (aa) in length. It contains one large catalytic site (aa 24-462), and typically functions as a noncovalent homodimer. There are at least two potential isoform variants. One contains a three aa substitution for aa 425-464, while another possesses a 22 aa substitution for aa 302-464. Over aa267-464 mouse Kynu shares 91% and 80% aa sequence identity with rat and human Kynu, respectively.
Alternate Names
KYNU, L-kynurenine hydrolase
Gene Symbol
KYNU
UniProt
Additional Kynureninase Products
Product Documents for Mouse/Rat Kynureninase Antibody
Product Specific Notices for Mouse/Rat Kynureninase Antibody
For research use only
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