Mouse Renin 1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF4277
Key Product Details
Species Reactivity
Mouse
Applications
ELISA Detection (Matched Antibody Pair), Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Renin 1
Specificity
Detects mouse Renin 1 in ELISAs and Western blots. In sandwich immunoassays, approximately 4% cross-reactivity with recombinant rat Renin is observed and less than 0.2% cross-reactivity with recombinant human Renin is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Renin 1 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Renin (Catalog # 4277-AS)
Sample: Recombinant Mouse Renin (Catalog # 4277-AS)
Mouse Renin 1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Renin
Mouse Renin is a secreted, 42‑47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin 1) is synthesized as a 381 aa proform (aa 22‑402). In the kidney, pro‑Renin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro Renin shares 70% and 85% aa sequence identity with human and rat pro Renin, respectively.
Additional Renin Products
Product Documents for Mouse Renin 1 Biotinylated Antibody
Product Specific Notices for Mouse Renin 1 Biotinylated Antibody
For research use only
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