Mouse S100A16 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6397
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E.coli-derived recombinant mouse S100A16
Ala2-Ser124
Accession # NP_080692
Ala2-Ser124
Accession # NP_080692
Specificity
Detects mouse S100A16 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human S100A16 is observed, and less than 1% cross-reactivity with recombinant mouse S100A4, recombinant human (rh) S100A2, and rhS100A10 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse S100A16 Antibody
Detection of Mouse S100A16 by Western Blot.
Western blot shows lysates of mouse brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Mouse S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6397) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A16 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse S100A16 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse brain (cerebellum) tissue
Sample: Mouse brain (cerebellum) tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A16
Mouse S100A16 (S100 calcium-binding protein A16; also S100F and AAG13) is a 15-18 kDa member of the S100 family of calcium-binding proteins. It is found in both cytoplasm (when Ca is high) and nucleus (when Ca is low), and its expression appears to be restricted to astrocytes found in select areas of the brain. S100A16 is 124 amino acids (aa) in length. Based on human, it will contain an N-terminal non-Ca-binding EF-hand domain (aa 12-47), a C-terminal Ca-binding EF-hand domain (aa 54-89), and a putative FGF-1 binding site (aa 91-94). There is one potential myristoylation site (aa 54-59) and a phosphorylation site at Ser124. S100A16 will homodimerize, and perhaps form higher order oligomers. Full length mouse S100A16 shares 95% aa identity with rat S100A16. Relative to human S100A16, mouse S100A16 possesses a 21 aa Glu/Gln-rich insertion after Glu98 that reduces the shared aa homology between mouse and human S100A16 to 72% aa identity.
Long Name
S100 Calcium Binding Protein A16
Alternate Names
AAG13, DT1P1A7, S100F
Gene Symbol
S100A16
UniProt
Additional S100A16 Products
Product Documents for Mouse S100A16 Antibody
Product Specific Notices for Mouse S100A16 Antibody
For research use only
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