Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE-conjugated Monoclonal Antibody (Catalog # FAB1356P) and either (A) Rat Anti-Mouse Sca-1/Ly6 Monoclonal Antibody (Catalog # MAB1226) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Sca-1/Ly6 in Mouse Splenocytes.

Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse Sca-1/Ly6 Monoclonal Antibody (Catalog # MAB1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse Sca-1/Ly6 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse splenocytes gated on hematopoietic lineage negative cells

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sca-1/Ly6

Stem Cell Antigen-1 (Sca-1) is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

References

Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634.
Spangrude, G.I. et al. (1988) Science 241:58.
Spangrude, G.I. et al. (1993) Blood 82:3327.
Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
Yamamoto, Y. et al. (1996, Blood 88:445.
Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
Welm, B.E. et al. (2002) Dev. Biol. 245:42.
Codias, E.K. et al. (1990) J. Immunol. 144:2197.
Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
Codias, E.K. et al. (1990) J. Immunol. 145:1407.
Flood, P.M. et al. (1990) J. Exp. Med. 172:115.

Long Name

Stem Cell Antigen-1/Lymphocyte Antigen 6

Alternate Names

Ly-6, Ly6, Sca1

Entrez Gene IDs

110454 (Mouse)

Gene Symbol

LY6A

UniProt

CAA28351

Additional Sca-1/Ly6 Products

Product Documents for Mouse Sca-1/Ly6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Sca-1/Ly6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Sca-1/Ly6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1226

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Sca-1/Ly6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Sca-1/Ly6 Antibody

Detection of Sca-1/Ly6 in Mouse Splenocytes by Flow Cytometry.