Mouse Serpin E1/PAI-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3828
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunoprecipitation, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin E1/PAI-1
Leu24-Ser402
Accession # AAH54091
Leu24-Ser402
Accession # AAH54091
Specificity
Detects mouse Serpin E1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Serpin E1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Serpin E1/PAI-1 Antibody
Detection of Mouse Serpin E1/PAI‑1 by Western Blot.
Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin E1/PAI-1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Serpin E1/PAI‑1 by Simple WesternTM.
Simple Western lane view shows lysates of mouse placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI-1 at approximately 56 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse Serpin E1/PAI-1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serpin E1/PAI-1, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serpin E1/PAI-1, see our available Western blot detection antibodies
Simple Western
12.5 µg/mL
Sample: Mouse placenta tissue
Sample: Mouse placenta tissue
Western Blot
0.25 µg/mL
Sample: Mouse placenta tissue
Sample: Mouse placenta tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin E1/PAI-1
Long Name
Plasminogen Activator Inhibitor
Alternate Names
Nexin, PAI-1, PAI1, PLANH1
Gene Symbol
SERPINE1
UniProt
Additional Serpin E1/PAI-1 Products
Product Documents for Mouse Serpin E1/PAI-1 Antibody
Product Specific Notices for Mouse Serpin E1/PAI-1 Antibody
For research use only
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