Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM4642
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA Detection (Matched Antibody Pair)
Cited:
ELISA Development
Label
Biotin
Antibody Source
Monoclonal Rat IgG2A Clone # 171028
Product Specifications
Immunogen
E. coli-derived recombinant mouse Sonic Hedgehog N-Terminus
Cys25-Gly198 (Lys122Arg)
Accession # Q62226
Cys25-Gly198 (Lys122Arg)
Accession # Q62226
Specificity
Detects mouse Sonic Hedgehog (Shh) N-terminal peptide in ELISAs. In ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant human (rh) Shh N-terminal peptide but no cross-reactivity with rmDhh N-terminal (aa 23‑198) or C‑terminal (aa 199‑396) peptides, rmShh C-terminal peptide (aa 199-437), or rmHIP.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody
Application
Recommended Usage
Mouse Sonic Hedgehog Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sonic Hedgehog/Shh
Shh is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.
Alternate Names
HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS
Gene Symbol
SHH
UniProt
Additional Sonic Hedgehog/Shh Products
Product Documents for Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody
Product Specific Notices for Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody
For research use only
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