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Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM4642

R&D Systems, part of Bio-Techne
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BAM4642

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # 171028

Product Specifications

Immunogen

E. coli-derived recombinant mouse Sonic Hedgehog N-Terminus
Cys25-Gly198 (Lys122Arg)
Accession # Q62226

Specificity

Detects mouse Sonic Hedgehog (Shh) N-terminal peptide in ELISAs. In ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant human (rh) Shh N-terminal peptide but no cross-reactivity with rmDhh N-terminal (aa 23‑198) or C‑terminal (aa 199‑396) peptides, rmShh C-terminal peptide (aa 199-437), or rmHIP.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody

Mouse Sonic Hedgehog Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse Sonic Hedgehog/Shh N-Terminus Antibody (Catalog # MAB4641)
  • Standard: Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein (Catalog # 461-SH)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sonic Hedgehog/Shh

Shh is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.

Alternate Names

HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS

Entrez Gene IDs

6469 (Human); 20423 (Mouse)

Gene Symbol

SHH

UniProt

Additional Sonic Hedgehog/Shh Products

Product Documents for Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody

For research use only

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